Premium Analysis ServiceRNA-Seq & Agilent Microarray experiments

Do you have experiences of getting odd results?

We have provided data analysis services for long, and we know customers sometimes bring a data set which is impossible to analyze, or terribly bad quality. They did not consider the experimental plan, but it is impossible to recover once they have done.

  1. Experimental Plan
    They did not have a concrete image about the data and the way of analysis.
  2. Experiment
    They somehow selected the platform without assessing by themselves.
  3. Data Analysis
    They finally found they could not analyze the data.

Backward Thinking Helps

The "backward thinking" is important to avoid such a waste of time and money.
We recommend you take time to consider the experimental plan before you actually do.

  1. Data Analysis
    You have a clear image about the data and the way of analysis.
  2. Experiment
    You assess platforms to select the right one with the capability.
  3. Experimental Plan
    You strategically allocate resources to achieve at the limited budget.

We have supported many researchers at the data analysis part, and sometimes they brought terribly bad quality data. Unfortunately, we cannot do anything, because the data analysis is not a magic.

They could have avoided it, if they had considered the following points very well.

  1. Have a good experimental design.
  2. Select a capable platform.
  3. Have it done by a skillful technician.

Maybe it is easy to understand the third point. But it is difficult to think about the first or second, especially if you do not have a lot of experience.

So Subio provides the service to have you make an adequate experimental plan by showing you the real researchers' data sets. You send the sample of tissues or cells, and our skillful technicians extract RNA and measure by microarray or RNA-Seq.

We deliver the raw data and analysis results in a SSA(Subio Series Archive) file, so that you can visually understand how the raw signal distributed, how the normalization/pre-processing was applied and how the analysis performed. We hold a web meeting to explain about the characteristics of raw data, and why we chose those analysis steps. Even after the meeting, you can request online support sessions every time you need a help or consulting.

We have over 10 years' experience of providing data analysis service, and this is why we know many customers, especially who get data from service providers or facilities, have problems on raw data or analysis workflows. And they use the data without noticing about the poor quality.

This is why we started experiment service, even though we do not have the facility. We will stand between you and a highly skillful and trustful facility to provide a service;

  1. We do not hide the bad quality.
  2. We tell you the quality of each sample, even if it is bad, not to mislead your interpretation and conclusion. And maybe we will advise you for future experimental design. Also we check the quality to prevent your accepting data containing defects.

  3. We do not tell as is you can do all.
  4. We help your assessment to understand the limitation of the technology. With a clear image about what you can do and what you cannot do is the basement of your experimental planning. And it is also true that omics experiment produces bad samples at a certain rate. So we advise risk hedge options on your experimental plan.

  5. We do not advise "more" replicates.
  6. Of course, you know more replicates is better. But you actually have a limit in budget, sampling, human resource and time. And you know that it is much better to have one good sample than triplicated unstable samples. What is important is to prioritize your goals and strategically allocate replicates on samples. Otherwise, you will lose your money and time in vain.

We have sublimated our experience and knowledge to Premium Analysis Service.

Features of Premium Analysis Service

  • We consistently support from experimental design to data analysis.
  • We suggest methods of experiment and data analysis to achieve the goal.
  • We suggest experimental plans within the budget, which are expandable in the future.
  • Skillful technicians take in charge of the experiment to generate the best quality raw data.
  • We preserve the aliquots, and you can order RT-PCR experiment for validation.
Price
RNA-Seq or Microarray experiment 1,200 USD / Sample
Advisory service fee * 280 USD
* Advisory service includes consultation of experimental planning, quality check of raw data, data analysis,
explanation in a web meeting.
Price
RNA-Seq or Microarray experiment 1,200 EUR / Sample
Advisory service fee * 280 EUR
* Advisory service includes consultation of experimental planning, quality check of raw data, data analysis,
explanation in a web meeting.
Price
RNA-Seq or Microarray experiment 120,000 JPY / Sample
Advisory service fee * 28,000 JPY
* Advisory service includes consultation of experimental planning, quality check of raw data, data analysis,
explanation in a web meeting.

Other NGS Experiment Services

The price will be determined upon your request. Please contact us for more information.

  • Small volume RNA-Seq *1
  • Degraded RNA-Seq *2
  • Small volume + degraded RNA-Seq *1 *2
  • lncRNA (long non-coding RNA) sequencing
  • small RNA sequencing
  • microRNA sequencing
  • Amplicon sequencing
  • De Novo genome sequencing
  • Metagenomic sequencing
  • 16s rRNA sequencing
  • *1 we accept 100~1000 cells as input.
  • *2 we accept sorted and selected cells, pathological specimens, laser microdissection tissues or something from which you cannot get the good quality RNA.

Inquiry

If you have any questions, please send a message from the following form. We accept message in English or Japanese.

* required

Please zip all files and upload the zip file.

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