Importing RNA-Seq FASTQ files into Subio Platform means to run a pipeline composed of fastp , HISAT2, and StringTie . But you don't need a workstation nor UNIX command-line skills. It works on an ordinary Windows or Mac computer. This is the easiest and cheapest way to build an RNA-Seq data processing environment on the Windows system. Even for who can use the command-line tools, doing it with the Subio Platform is more comfortable.
Before you run the pipe, you have to install and setup these tools. If you have any difficulties in the installation, please order the Troubleshooting Service on FASTQ processing.
Instruction - How to import RNA-Seq FASTQ files
Have the FASTQ files to import as .gz archived files. It generates intermediate results in the same folder, so be sure that there is enough free disk space, which is at least five times larger than the total size of the FASTQ files before you start executing. The process will stop in the middle if there is no disk space available. The FASTQ file directory can be on an external disk.
If you have paired-end FASTQ files, please follow the rule of file names. And if you get any errors, please try troubleshooting.
Importing RNA-Seq FASTQ files
After this, please go forward to the next tutorial of how to analyze the gene expression data by RNA-Seq.